Part:BBa_K5477006:Design
pPDC1 - Lex6Op - pENO1 bidirectional synthetic promoter with Lex6Op
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our system, we used the core region of the pPDC1 promoter with an emphasis on glucose-dependent regulation and the core region of pENO1. In our Lex6Op bidirectional system, pPDC1 serves as a promoter, working in tandem with another promoter (pENO1) to drive the expression of two different genes in opposite orientations. The Lex6Op operator cassette, composed of six tandem LexA operator sites, regulates the bidirectional activation of both promoters. When a LexA fusion protein (such as LexA-ERα, LexA-mERα or LexA-ERRγ) binds to the Lex6Op sequence, it activates transcription through both pENO1 and pPDC1, enabling the coordinated expression of two genes simultaneously.
Source
The core promoters are from Saccharomyces cerevisiae<i/>
===References===